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重大技术突破！朱健康院士团队等合作开发无需组培的植物遗传转化技术

花匠小妙招
2025-10-14 20:29

近日，The innovation(即时IF=40.76)在线发表了朱健康院士团队等合作的题为“Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture”的研究论文。 该论文报道了一种无需组织培养等过程即可方便快速获得转基因及基因组编辑植株的方法。

该研究介绍了一种极其简单的切-浸-芽（cut-dip-budding，CDB）系统，该系统利用发根农杆菌侵染切开后的根茎交界处，进而产生转化根，再通过根转化产生转化芽。该方法功实现多个植物物种的遗传转化，包括两种草本植物(Taraxacum kok-saghyz and Coronilla varia)、一种块根植物( sweet potato )和三种木本植物( Ailanthus altissima, Aralia elata, and Clerodendrum chinense）。在此之前，这些植物均是很难或不可能转化的，CDB方法能够在非无菌条件下，在无需组织培养的情况下，使用非常简单的外植体侵染方法对其进行高效转化或基因编辑。

Figure 1. The CDB delivery system and genetic transformation of TKS (A) CDB delivery system workflow. Seedlings that were 3 to 4 weeks old were cut and used as explants. The reporter gene or gene-editing constructs were delivered to plant cells near the cut site via A. hizogenes. Hairy roots were formed after several weeks. The GFP-positive roots were segmented and cultured to give rise to transgene-positive or gene-edited shoots. (B) A 3- to 4-week-old seedling. (C) Explant. Gray arrow indicates the site of infection. (D) Explants inoculated with Agrobacterium were cultured in vermiculite. (E and F) Formation of GFP positive hairy root of TKS. (G and H) Positive hairy roots continued to develop. (I and J) GFP-positive root segment. (K and L) Transgene-positive shoots formed on a GFP-positive root segment. (M) Transformed shoots that were planted into soil grew normally to the reproductive stage. In (E, F, I, J, L and I) the sample on the left is untransformed control. In (F, H, J and I) samples were illuminated with fluorescent light. Red arrow indicates GFP fluorescence in transformed tissue.

Figure 2. Gene editing in TKS using the CDB deliver system. (A) Schematic of the TKS gene-editing vector. (B) A shoot that was formed on a wild-type root segment. (C) Albino phenotype of a homozygous TkPDS knockout shoot. (D) The CDB-delivery system efficiently delivered gene-editing tools and generated edited shoots in three independent experiments. (E) Sequencing results of a representative TkPDS knockout albino seedling. The sgRNA target sequence is shown in orange, the protospacer-adjacent motif (PAM) is shown in blue, and mutations are shown in red. Bar = 1 cm.

Figure 3. CDB-mediated gene editing in sweet potato. (A) Illustration of the CDB delivery process in sweet potato. Transgenes were delivered by A. rhizogene to sweet potato apical stem tissue, resulting in the induction of hairy roots under non-sterile conditions. After approximately 10 weeks, some of these hairy roots developed into tuberous roots. Transgenic (GFP-positive, indicated in brown color) roots (including tuberous roots) were harvested and allowed to bud. Transgene-positive shoots formed were transplanted into soil. (B) Photograph of an explant used in the experiment; gray arrow points to the site of infection by A. rhizogene. (C) Inoculated explants were inserted into vermiculite and cultured under a high-moisture environment. (D and E) Transgenic hairy roots formed. (F and G) Hairy roots were allowed to grow and some developed into tuberous roots. (H and I) GFP-positive roots were cut into segments. (J) Transgene-positive shoots formed on a root fragment. (K) Shoots formed on a wild-type root segment, (L) Albino shoots of a homozygous PDS knockout mutant. (M) CDB delivery system efficiently delivered gene-editing tools into sweet potato and generated edited shoots in three independent experiments. (N) Sequencing of the target region in PDS in an albino plant. The sgRNA target sequence is shown in orange, the protospacer-adjacent motif (PAM) is shown in blue, and mutations are shown in red. Red arrows in e, g and i point to GFP-positive signal. Bar = 1cm

Figure 4. CDB delivery system is genotype-independent in sweet potato.(A) GFP was transformed into 10 sweet potato cultivars via the CDB delivery system. (B) The number of transgenic shoots obtained from each of the 10 sweet potato cultivars. (C) Albino shoots indicating homozygous PDS knockout in the Xuzishu8 cultivar. (D) Sequencing results of the albino shoot of Xuzhishu8. Bar = 1cm.

Figure 5. CDB delivery system was effective in transforming three woody plant species and a leguminous pasture plant. (A-D) Transformation of Ailanthus altissima (Mill) Swingle (Aa) via the CDB system. (A) Hairy root formation on Aa explants. (B) Hairy roots of Aa developed into normal-appearing roots. (C) Segments of transgene (RUBY)-positive Aa roots. (D) Transgene-positive shoot formed on an Aa root segment. (E-G) Transformation of Aralia elata Seem. (Ae) via the CDB system. (E) Hairy root formation and development into normal-appearing roots of Ae. (F) Segments of transgene (GFP)-positive Ae roots. (G) Transgene-positive shoot formed on an Ae root segment. (H and I) Transformation of Clerodendrum chinense Mabb. (Cc) via the CDB system. (J and K) Transformation of Coronilla varia L. (Cv) via the CDB system. (L) Statistics for the three woody plant species (Aa, Ae, and Cc) and the leguminous pasture plant Cv transformed via the CDB delivery system. Red arrow indicates transformed tissue. Bar = 1 cm.

原文链接：

https://doi.org/10.1016/j.xinn.2022.100345

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