首页 > 分享 > 响应面优化西兰花中萝卜硫素复合提取工艺

响应面优化西兰花中萝卜硫素复合提取工艺

花匠小妙招
2024-11-18 00:05

摘要: 以西兰花鲜食部分绿色幼嫩花茎和花蕾为实验材料,利用响应面对添加剂和超声波辅助酶解西兰花提取萝卜硫素工艺进行优化。实验在单因素基础上选取四种添加剂抗坏血酸、EDTA、Mg SO4、CaCl2,根据Central Composite中心复合实验设计原理采用四因素三水平的响应面分析法,确定各因素对萝卜硫素提取率的影响程度,获得该四因素最佳提取条件为:抗坏血酸4.659 mmol/L,EDTA 6 mmol/L,Mg SO4 8.5 mmol/L,Ca Cl24.0 mmol/L,萝卜硫素理论最大提取率达1858.20μg/g。经实验验证达1813.26μg/g,其相对误差为2.48%,表明该提取工艺参数在西兰花中提取萝卜硫素是可行的。

关键词: 萝卜硫素 / 酶解 / 添加剂 / 单因素 / 响应面

Abstract: The green young stems and buds of broccoli were used as experimental material,the enzymolysis condition of sulforaphane extraction from broccoli was optimized by response surface method. According to central composite experiment design principle and using response surface analysis of four factors and three levels,four additives including ascorbic acid,EDTA,Mg SO_4,Ca Cl_2 were chosen on the basis of the single factor to determine the impact of various factors on the extraction rate of sulforaphane. The optimum extraction conditions were as follows:ascorbic acid 4.659 mg/g,EDTA 6 mmol/L,Mg SO_4 8.5 mmol/L,Ca Cl_2 4.0 mmol/L.The theoretical maximum extraction rate of sulforaphane was 1858.20 μg/g,and the value reached 1813.26 μg/g in the experiment,the relative error was 2.48%,which indicated that the extraction process parameter above was feasible in sulforaphane extraction from broccoli.

[1]

Madhu,Anita K.Proximate composition,available carbohydrates,dietary fibre and anti-nutritional factors of Broccoli(Brassica oleracea L.Var.Italica Plenck)leaf and floret powder[J].Bioscience Discovery,2014,5(1):45-49.

[2]

Meerte H,Peter M,Hilmer S,et al.Glucosinolates in broccoli stored under controlling Atmosphere[J].J Ameri Soc Hort Sci,1995,120(6):1069-1074.

[3]

Yardfon T,Shyam S S,Naphaporn C,et al.Microwave-assisted extraction of Sulforaphane from white cabbages:Effects of extraction condition,solvent and sample pretreatment[J].Journal of Food Engineering,2013,117(1):151-157.

[4]

Huang T Y,Chang W C,Wang M Y,et al.Effect of sulforaphane on growth inhibition in human brain malignant glioma GBM8401 cells by means of mitochondrialand MEK/ERK-Mediated apoptosis pathway[J].Cell Biochem Biophys,2012,63:247-259.

[5]

You Y R,Wu Y F,Mao J W,et al.Screening of Chinese brassica species for anti-cancer sulforaphane and erucin[J].African Journal of Biotechnology,2008,7(2):147-152.

[6]

Monia L,Carmela F,Patrizia H.Sulforaphane as a promising molecule for fighting cancer[J].Mutation Research/Reviews in Mutation Research,2014,159:207-223.

[7] 王见冬,袁其朋,钱忠明.萝卜硫素研究进展[J].食品与发酵工业,2003,29(2):76-80. [8]

Han D D,Row K H.Separation and purification of sulforaphane from broccoli by solid phase extraction[J].International Journal of Molecular Sciences,2011,12:1854-1861.

[9]

Luca L,Giovanna C,Onofrio L,et al.Effects of glucosinolates and their enzymatic hydrolysis products myrosinase on the rootknot nematode Meloidogyne incognita(Kofoid et White)Chitw[J].Agriculture of Food Chemistry,2004,52(22):6703-6707.

[10] 程坤,杨丽梅,方智远,等.十字花科植物中主要硫代葡萄糖苷合成与调节基因的研究进展[J].中国蔬菜,2010(12):1-6. [11]

Bones A,Slupphaug G.Purification,characterization and partial amino acid sequencing ofβ-thioglucosidase from Brassica napus L.[J].Journal Plant Physiology,1989,134(6):722-729.

[12] 周晨光,朱毅,罗云波.十字花科蔬菜萝卜硫素含量的影响因素[J].食品工业科技,2013,34(12):371-381. [13] 黄肖钦,马朱莉,李娜,等.西兰花种子酶解生成萝卜硫素的条件研究[J].上海工程技术大学学报,2010,24(3):277-280. [14]

Yuan H N,Yao S J,You Y R,et al.Antioxidant activity of isothiocyanate extracts from broccoli[J].Chinese Journal of Chemical Engineering,2010,18(2):312-321.

[15]

Wu Y F,Mao J W,You Y R,et al.Study on degradation kinetics of Sulforaphane in broccoli extract[J].Food Chemistry,2014,155:235-239.

[16] 李娟,朱祝军.植物中硫代葡萄糖苷生物代谢的分子机制[J].细胞生物学杂志,2005,27:519-524. [17]

Ishikawa T,Okazaki K,Kuroda H,et al.Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development[J].Molecular Plant Pathology,2007,8(5):623-637.

[18]

Maik K,Dirk S.A novel approach for reliable activity determination of ascorbic acid de pending myrosinases[J].Journal of Biochemical and Biophysical Methods,2004,59(3):253-265.

[19]

Burmeister W P,Cottaz S,Rollin P,et al.High resolution X-ray crystallography shows that is a cofactor for myrosinase and substitutes for the function of the catalytic base[J].Journal of Biological Chemistry,2000,275(50):39385-39393.

[20]

Ludikhuyze L,Rodrigo L,Hendrickx M.The Activity of Myrosinase from Broccoli(Brassica oleracea L.cv.Italica):Influence of Intrinsic and Extrinsic Factors[J].Journal of Food Protection,2000,63(3):400-403.