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甜瓜果皮颜色遗传分析及基因定位

花匠小妙招
2024-12-16 22:30

为探究甜瓜皮色性状的遗传规律，定位目标性状关联的遗传位点，选用黄皮材料B432 和绿皮材料B168、B421 为亲本，构建6 世代遗传群体(P1、P2、F1、F2、BC1P1、BC1P2)，用于分析甜瓜皮色性状的遗传规律；同时，利用BSA-seq 和全基因组重测序技术，定位控制甜瓜皮色的基因。结果表明，甜瓜皮色由2对基因控制，其中绿色对白色具有显性上位性效应，白色对黄色为显性。同时，将皮色相关基因分别定位于4 号染色体和10 号染色体的0.02~5.7 Mb和0.08~9.5 Mb区间。试验初步定位了控制甜瓜皮色的基因，为后续进行基因精细定位提供依据，为开展甜瓜皮色的分子标记辅助选择奠定基础。

Abstract

To explore the inheritance and genetic loci of melon rind color, the yellow-rind line B432 and green-rind lines B168 and B421 were used as parent to construct six generations of genetic population (P1, P2, F1, F2, BC1P1, BC1P2) to analyze the inheritance of melon rind color. In addition, the BSA-seq and whole genome resequencing technology were used to locate the genetic loci responsible for melon rind color. The results show that the melon rind color is controlled by two genes, of which green has a dominant epistatic effect on white and white is dominant to yellow. The rind color related genes are mapped to the 0.02-5.7 Mb and 0.08- 9.5 Mb intervals of chromosome 4 and chromosome 10, respectively. This experiment preliminarily elucidates the genetic mechanism and the location of the genes controlling the rind color of melon, and could provide a basis for the subsequent fine-grained gene mapping and molecular marker-assisted selection of melon rind color.

关键词

甜瓜；果皮颜色；遗传分析；基因定位；BSA{{custom_keyword}} /

Key words

melon; rind color; genetic analysis; gene mapping; BSA{{custom_keyword}} /

欧点点,赵光伟,贺玉花,王平勇,徐志红,孔维虎,张健,徐永阳.甜瓜果皮颜色遗传分析及基因定位. 中国农学通报. 2019, 35(13): 64-69 https://doi.org/10.11924/j.issn.1000-6850.casb18110127

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