费菜不同组织总黄酮抗氧化与抑菌活性的研究
摘要： 本文采用费菜不同组织（嫩叶、老叶、茎、花）中总黄酮进行体外抗氧化能力及抑菌作用的研究。对Fe3+还原活性、清除DPPH·、·OH进行体外抗氧化活性试验，并采用两倍稀释法探讨费菜不同组织抑制大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的活性。结果表明，费菜不同组织总黄酮的Fe3+还原活性、DPPH·清除率、·OH清除率均显著高于L-抗坏血酸。其中，Fe3+还原活性可达到810 μmol TE/mL以上；对DPPH·以及·OH清除率的IC50值最低分别为0.8 μg/mL（茎）和14 μg/mL（花）。费菜各组织总黄酮均有一定的抑菌作用，对金黄色葡萄球菌的抑制效果较好；对于大肠杆菌，茎和嫩叶中的总黄酮的抑制效果明显比老叶和花强；而四个组织总黄酮对枯草芽孢杆菌的抑制作用相当，其中老叶总黄酮的抑制效果略好。该试验结果为费菜不同组织总黄酮的应用提供理论基础。
关键词: 费菜, 黄酮, 抗氧化, 抑菌活性
Abstract: The antioxidant and antibacterial activities of total flavonoids from different parts of Sedum aizoon L were studied in this paper.Antioxidant activities of total flavonoids from tender leaves,old leaves,stems and flowers of Sedum aizoon L were evaluated by reducing power on Fe3+,DPPH and Hydroxyl Radical scavenging activity assays. Escherichia coli,Staphylococus aureus and Bacillus subtilis were used as experimental strains,the antibacterial activity of different parts were determined by the method of double dilution tests.The results showed that the Ferric Reducing Antioxidant Power,DPPH and Hydroxyl Radical scavenging activity of total flavonoids in different parts of Sedum aizoon L.were significantly higher than L-ascorbic acid.The Ferric Reducing antioxidant power of total flavonoids in different parts of Sedum aizoon L.could reach up to 810 μmol TE/mL and the IC50 values of DPPH free radical clearance and Hydroxyl Radical scavenging activities for stems and flowers were 0.8 and 14 μg/mL,respectively.The total flavonoids in various parts of Sedum aizoon L.had certain antibacterial effect,and the inhibitory effect on Staphylococcus aureus was better.The antibacterial effect of total flavonoids in different parts had good inhibitory effect on Staphylococcus aureus;The antibacterial effect of total flavonoids in stems and tender leaves to Escherichia coli was significantly better than old leaves and flowers;While the antibacterial effect of total favonoids in the old leaves to Bacillus subtilis was a little better than other parts.The results can provide a scientific basis for the utilization of total flavonoids from different parts of Sedum aizoon L.
Key words: Sedum aizoon L., flavonoid, antioxidant, antimicrobial activity
中图分类号:
R284Q946
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