火龙果花的体外抗氧化物提取工艺优化及其抗炎活性
张艳军, 廖日权, 郑云云, 尹艳镇, 黄秋园, 徐国鑫, 黄恩交, 朱靖清, 吴彩丽. 火龙果花的体外抗氧化物提取工艺优化及其抗炎活性[J]. 食品工业科技, 2018, 39(18): 137-142. DOI: 10.13386/j.issn1002-0306.2018.18.025
引用本文: 张艳军, 廖日权, 郑云云, 尹艳镇, 黄秋园, 徐国鑫, 黄恩交, 朱靖清, 吴彩丽. 火龙果花的体外抗氧化物提取工艺优化及其抗炎活性[J]. 食品工业科技, 2018, 39(18): 137-142. DOI: 10.13386/j.issn1002-0306.2018.18.025
ZHANG Yan-jun, LIAO Ri-quan, ZHENG Yun-yun, YIN Yan-zhen, HUANG Qiu-yuan, XU Guo-xin, HUANG En-jiao, ZHU Jing-qing, WU Cai-li. Optimization of Extraction Technology on Antioxidants in Vitro from Pitaya Flower and Evaluation of Anti-inflammatory Activities[J]. Science and Technology of Food Industry, 2018, 39(18): 137-142. DOI: 10.13386/j.issn1002-0306.2018.18.025
Citation: ZHANG Yan-jun, LIAO Ri-quan, ZHENG Yun-yun, YIN Yan-zhen, HUANG Qiu-yuan, XU Guo-xin, HUANG En-jiao, ZHU Jing-qing, WU Cai-li. Optimization of Extraction Technology on Antioxidants in Vitro from Pitaya Flower and Evaluation of Anti-inflammatory Activities[J]. Science and Technology of Food Industry, 2018, 39(18): 137-142. DOI: 10.13386/j.issn1002-0306.2018.18.025
火龙果花的体外抗氧化物提取工艺优化及其抗炎活性
详细信息
作者简介:张艳军(1980-),女,硕士,研究方向:天然产物活性物质的提取、分离,E-mail:zhangyj201608@163.com。
中图分类号: TS201.2
计量 文章访问数: 0237 HTML全文浏览量: 047 PDF下载量: 08 出版历程 收稿日期: 2017-12-13 网络出版日期: 2020-11-16 刊出日期: 2018-09-14Optimization of Extraction Technology on Antioxidants in Vitro from Pitaya Flower and Evaluation of Anti-inflammatory Activities
Guangxi Colleges and Universities Key Laboratory of Beibu Gulf Oil and Natural Gas Resource Effective Utilization, College of Chemistry and Chemical Engineering, Qinzhou University, Qinzhou 535011, China
摘要
摘要: 旨在为探究火龙果花的抗氧化物提取工艺及其抗炎活性。选取火龙果花为原料,用热回流法提取,以粗提液对DPPH自由基及OH自由基的清除能力为指标,先进行单因素实验,考察提取温度、料液比、提取时间以及乙醇浓度对粗提物抗氧化活性的影响,再采用L9(34)正交试验法,优化火龙果花的体外抗氧化物提取工艺。结果表明:70%乙醇,1：30 g/mL料液比,75 ℃下回流加热3.0 h为最优提取条件,在最佳提取工艺下粗提物对DPPH自由基、OH自由基的IC50值分别为0.273、0.288 mg/mL,且在一定范围内,粗提物的浓度越高,其抗氧化活性越好,量效关系明显。最佳工艺条件下,粗提物对LPS诱导RAW264.7巨噬细胞产生的NO有很强的抑制作用,且与浓度呈正相关,具有剂量依赖性,其半抑制浓度IC50值为13.94 μg/mL,该提取物具有很好的抗炎活性。
Abstract: To investigate the optimum technology for extraction of antioxidants and the extracts' anti-inflammatory activity from pitaya flower, the antioxidant activity in vitro of the extracts from pitaya flower was determined by scavenging capacity of DPPH·and·OH.The effects of extraction temperature, solid-liquid ratio, extraction time and ethanol concentration were studied through the single factor experiments, and L9 (34) orthogonal test was used to explore the antioxidant activities of extracts from pitaya flower in vitro. The results showed that, the optimized conditions were:70% ethanol, the solid-liquid ratio of 1:30 g/mL, when treatment time was 3.0 h at 75℃. Under these optimized conditions, the IC50 for scavenging capacity of the extracts against DPPH·and·OH were 0.273, 0.288 mg/mL, respectively. And within a certain range, the higher the concentration of the crude extract, the better its antioxidant activity, which had an obviously dose-effect relationship. Moreover the extracts under the optimized conditions could decrease secretions of NO in LPS-induced RAW264.7 macrophage cells, and the anti-inflammatory activity for the extracts increased in a concentration-dependent manner with IC50 value of 13.94 μg/mL. The extracts had good antioxidant activity and anti-inflammatory activity in vitro.
HTML全文
参考文献(0)
施引文献
资源附件(0)
计量 文章访问数: HTML全文浏览量: PDF下载量: 出版历程 收稿日期: 2017-12-13 网络出版日期: 2020-11-16 刊出日期: 2018-09-14目录
相关知识
响应面法优化有机溶剂提取火龙果花精油工艺的研究
酶法辅助提取迷迭香精油及其抗炎作用
微波辅助提取火龙果花多糖的工艺研究
向日葵茎芯多糖的提取工艺优化及其体外抗肿瘤活性研究
溶剂法提取火龙果籽油工艺优化及其理化性质
枇杷花茶水提物的镇咳、抗炎与抗氧化作用研究
桂花总黄酮的提取及其抗氧化活性研究
火龙果花中黄酮类化合物抗氧化活性研究
火龙果花化学成分的研究进展
食用花卉中多酚化合物的种类及其抗氧化性研究进展
网址: 火龙果花的体外抗氧化物提取工艺优化及其抗炎活性 https://m.huajiangbk.com/newsview1412542.html
| 上一篇: 加工工艺对茶树花品质及抗氧化活性 |
下一篇: 双氧水养花有这么多好处？我读的书 |
