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菊苣根中黄酮的提取工艺.doc

花匠小妙招
2025-05-02 02:29

摘要菊苣为多年生草本植物，广泛分布亚洲、美洲醇浓度％，提取时间，比为对影响最大的是和，其次是菊苣 Abstract Chicory is the perennial herb, mainly from Europe, now widely distributed in Asia, the America. Chicory root contains protein, carbohydrates, fats, flavonoids, cellulose and other nutrients, high flavonoid content, flavonoids are a class of phenolic compounds, benzene chromone ring has hepatoprotective, antihypertensive, diuretic and other effects. With the method of single factor experiments, this experiment applied ultrasonic ethanol extraction and hot water reflux extraction method flavonoids, by changing the extraction temperature, ethanol concentration, extraction time and liquid to solid ration in chicory root extract obtained under UV spectrophotometer measured absorbance by the absorbance, the results shows that hot water reflux extraction method obtained a higher flavonoid content, and final optimized conditions for extracting flavonoids was established, extracted 2.5 hours on 80℃, ethanol concentration 70% and solid to liquid ration 1:20. Then the orthogonal experiment to optimize the hot water reflux extraction method, and get to influence the flavonoid content is the extraction temperature and ethanol concentration, followed by the extraction time, solid to liquid ratio, the flavonoid content is 7.74mg / g under this optimum conditions. Keywords: chicory root；flavone；extract；orthogonality目录摘要I AbstractII 第一章前言1 第二章文献综述3 2.1 菊苣的简介3 2.1.1菊苣叶简介3 2.1.2 菊苣根简介4 2.2 总黄酮的简介5 2.2.1 总黄酮的理化性质5 2.2.2 总黄酮的功效成分5 2.2.3总黄酮的营养价值6 2.3菊苣根中总黄酮的提取方法7 2.3.1超声波乙醇提取法7 2.3.2乙醇回流提取法8 2.3.3 微波辅助提取法8 2.3.4 超临界提取法8 2.3.5 加速溶剂提取法9 2.4 菊苣根中总黄酮的测定方法9 2.4.1亚硝酸钠－硝酸铝－氢氧化钠比色法9 2.4.2紫外分光光度法9 2.4.3高效液相色谱法10 2.5 菊苣根总黄酮的研究目的与意义10 2.5.1 菊苣根总黄酮的研究目的10 2.5.2 菊苣根总黄酮的研究意义10 第三章实验部分12 3.1 实验样品12 3.2 实验仪器与试剂12 3.2.1实验仪器12 3.2.2 实验试剂12 3.3 实验方法13 3.3.1 菊苣根中总黄酮提取工艺13 3.3.2 菊苣根中总黄酮提取机理13 3.3.3 芦丁标准曲线14 3.3.4 菊苣根中总黄酮提取方法15 3.3.5 总黄酮的测定—分光光度法16 第四章结果与讨论17 4.1 芦丁标准曲线的绘制17 4.2 菊苣根中总黄酮提取结果18 4