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带叶兜兰种子原地共生萌发及有效菌根真菌的分离与鉴定.pdf全文

花匠小妙招
2024-11-23 10:38

带叶兜兰种子原地共生萌发及有效菌根真菌的分离与鉴定.pdf

热带亚热带植物学报　2015 ， 23(1) ： 59 ~ 64 Journal of Tropical and Subtropical Botany 带叶兜兰种子原地共生萌发及有效菌根真菌的分离与鉴定孙晓颖, 张武凡, 刘红霞* (北京林业大学林学院，北京 100083) 摘要：为获得带叶兜兰(Paphiopedilum hirsutissimum)种子萌发的共生真菌，采用原地共生萌发技术获得了 2 株自然萌发的小幼苗，并分离和筛选出了有效的种子萌发共生菌——瘤菌根菌(Epulorhiza sp.)。为验证分离菌株对带叶兜兰种子萌发的有效性，将 Phs34 号菌株与带叶兜兰种子在灭菌后的原生境基质上进行室内共生萌发试验，结果表明，经过 6 周的培养，对照组没有观察到种子的萌发；接菌的种子胚明显膨大，突破种皮，形成原球茎，平均萌发率为(58.35±3.41)%。这表明分离得到的瘤菌根菌能促进带叶兜兰的种子萌发。关键词：带叶兜兰；种子原地共生萌发；共生真菌；瘤菌根菌属 doi: 10.11926/j.issn.1005–3395.2015.01.009 In situ Symbiotic Seed Germination, Isolation and Identification of Effec- tive Mycorrhizal Fungus in Paphiopedilum hirsutissimum (Orchidaceae) * SUN Xiao-ying, ZHANG Wu-fan, LIU Hong-xia (College of Forestry, Beijing Forestry University, Beijing 100083, China) Abstract: The aim was to obtain the compatible mycorrhizal fungi of lithophytic Paphiopedilum hirsutissimum using in situ seed baiting technique. Two natural small seedlings were harvested after in situ seed germination. The effective symbiotic fungi of genus Epulorhiza were isolated from these developed seedlings. In order to verify the effect of the isolated fungal strains on seed germination, one of strains, Phs34, was used to the in vitro symbiotic seed germination of P. hirsutissimum on the sterile habitat substrate. After cultured for 6 weeks, seeds inoculated with Phs34 started to develop into protocorms and average germination rate was (58.35±3.41)%, whereas seeds without the fungus failed to germinate. These indicated that the fungal strain Phs34 was compatible mycorrhizal fungus of P. hirsutissimum, and it will be helpful in seedling production and orchid conservation of P. hirsutissimum. Key words: Paphiopedilum hirsutissimum; In situ symbiotic seed germination; Symbiotic fungi; Epulorhiza 兰科(Orchidaceae)是种子植物的一大家族，约数量巨大，一个蒴果内包含上万粒粉尘状的种子，有 800 属 25000~30000 种[1] 。目前，所有的野生兰然而兰花的种子胚发育不完全，没有胚乳