灵芝孢子副产物体外抗氧化活性的研究
摘要： 利用有机溶剂萃取法获得灵芝孢子副产物五个不同极性部位,以总抗氧化能力（T-AOC）及羟自由基（·OH）和2,2-二(4-叔辛基苯基)-1-苦肼基自由基（DPPH·）的清除能力为评价指标筛选最优抗氧化活性部位,并对其进行抗脂质过氧化研究,结果显示：不同极性部位均能清除上述自由基,其中乙酸乙酯相活性最强。进一步研究发现,乙酸乙酯相能够显著抑制β-胡萝卜素/亚油酸体系,同时对小鼠肝脏自氧化及H2O2诱导的肝脂质过氧化和红细胞过氧化溶血均有较好保护作用。实验表明：灵芝孢子副产物不同极性部位提取物均具一定程度的抗氧化活性,其中乙酸乙酯相活性最强。
关键词: 灵芝孢子副产物, 自由基, 乙酸乙酯相, 脂质过氧化
Abstract: The objective of this study was to investigate the antioxidant activities of by-products of Ganoderma lucidum spores.Ethanol soluble extract of by-products of Ganoderma lucidum spores was prepared by 80% alcohol,followed by fractional extraction respectively with petroleum ether,chloroform,ethyl acetate and n-butanol.Then the antioxidant activities of above five different fractions were evaluated by T-AOC and free radical scavenging power like DPPH· and ·OH to identify the most efficient fraction.The results indicated that all fractions showed high radical scavenging efficiency,especially the ethyl acetate fraction (EA) which was chosen to take a further study on anti-lipid peroxidation.In this study,the protective effects of EA at 0.5 mg/mL on β-carotene/linoleic acid system was 70.32%.The inhibitory activity of EA at 2 mg/mL on hemolysis of erythrocytes and lipid peroxidation of mice homogenate induced by H2O2 were 41.39% and 51.00%.Taking together,each extract has significant antioxidant activities,especially the ethyl acetate extract.
Key words: by-products of Ganoderma lucidum spores, free radicals, ethyl acetate extract, lipid peroxidation
中图分类号:
R285
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